Regulatory

Part:BBa_K578004:Design

Designed by: Ethan Ward   Group: iGEM11_Arizona_State   (2011-09-28)


Bacillus halodurans C-125 CRISPR leader sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has not been characterized in the literature. The sequence comprises the intergenic region between the CRISPR III locus and the Cas gene locus, which is AT rich as expected. The leader sequence for E. coli CRISPR I has been characterized and was used as a basis for this part.


Source

Synthesized by IDT. Genome coordinates 355127-355361.

References